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3 edition of Control of apoptosis in murine B cell hybridomas during stationary batch culture found in the catalog.

Control of apoptosis in murine B cell hybridomas during stationary batch culture

Joel Richard Charbonneau

Control of apoptosis in murine B cell hybridomas during stationary batch culture

by Joel Richard Charbonneau

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  • 34 Currently reading

Published by Laurentian University, Chemistry and Biochemistry Department in Sudbury, Ont .
Written in English


Edition Notes

Statementby Joel R. Charbonneau.
The Physical Object
Paginationxiv, 159 l. :
Number of Pages159
ID Numbers
Open LibraryOL20768101M
ISBN 100612612511
OCLC/WorldCa51000057

  Passaging of induced pluripotent stem cells in the automated culture device. In iPS cell culture, cell dissociation is the most important step. Usually, stem cell dispersion during manual Cited by: Apoptosis also occurs as a defence mechanism such as in immune reactions or when cells get damaged by disease or by noxious agents [2]. During the early process of apoptosis, cell shrinkage and .

Apoptosis or programmed cell death, is carefully coordinated collapse of cell, protein degradation, DNA fragmentation followed by rapid engulfment of corpses by neighbouring cells. (Tommi, ) Essential .   Apoptosis is a mode of cell death that is used by multicellular organisms to dispose of unwanted cells in a diversity of settings 1, many ways, what happens during apoptosis is akin to .

Define murine. murine synonyms, murine pronunciation, murine translation, English dictionary definition of murine. adj. 1. Of or relating to a rodent of the subfamily Murinae, which includes the house mouse . Cell Culture and Reporter Gene Assays—The murine B cell line A20 and human T cell line Jurkat were grown in RPMI medium supplemented with 10% fetal bovine serum, L-glutamine, sodium pyru .


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Control of apoptosis in murine B cell hybridomas during stationary batch culture by Joel Richard Charbonneau Download PDF EPUB FB2

Control of apoptosis in murine B cell hybridomas during stationary batch culture by Joel R. Charbonneau A thesis submitted in partial fulfillment of the requirement for the degree of Master of Science in. Control of apoptosis in murine B ce11 hybridomas duhg stationary batch culture by Joel R.

Charbonneau A thesis submitted in partial fùlfillrnent of the requirement for the degree of Master of Science in. Studies of bcl-2 expression in a Burkitts Lymphoma cell line and two studies of murine hybridomas indicated that bcl-2 substantially extended the duration of batch cultures by reducing the rate of cell Cited by: Apoptosis (from Ancient Greek ἀπόπτωσις, apóptōsis, "falling off") is a form of programmed cell death that occurs in multicellular organisms.

Biochemical events lead to characteristic cell changes and MeSH: D Apoptosis in cell culture. al-Rubeai M(1), Singh RP. This phenomenon, which has been named apoptosis, accounts for most of the cell deaths that take place during the production of Cited by: The cells of a multicellular organism are members of a highly organized community.

The number of cells in this community is tightly regulated—not simply by controlling the rate of cell division, but also by Cited by: The full text of this article hosted at is unavailable due to technical difficulties.

We were thinking to culture mouse B cells from the spleen in vitro for a week or two. We need them to expand and produce cytokines. I did some research on the protocols, many groups use feeder. Cell death is a fundamentally important problem in cell lines used by the biopharmaceutical industry.

Environmental stress, which can result from nutrient depletion, by-product accumulation and. In this context, forced expression of BHRF1, an Epstein–Barr virus-encoded early protein with structural and functional homology with the anti-apoptotic protein Bcl-2, effectively protected hybridomas in Cited by: 6.

AMINO ACID METABOLISM IN BATCH AND FED-BATCH CULTURE OF A MURINE MYELOMA CELL Jan Ljunggren and Lena Häggström Department of Biochemistry and Biotechnology, Royal Institute of Author: Jan Ljunggren, Lena Häggström.

Ishaque, A. and Al-Rubeai, M. () Role of Ca, Mg and K ions in determining apoptosis and extent of suppression afforded by bcl-2 during hybridoma cell culture. Apoptosis 4/5, – CrossRef Cited by: Apoptosis* (‘programmed cell death’) is a biologically ubiquitous phenomenon that deserves to be much more widely known among non-biologists and laypeople.

Put quite simply, without apoptosis, all. Presence of serum in the media has many drawbacks and can lead to serious misinterpretations in immunological studies [2, 3].A number of serum-free media have been developed [4, 5].These media Cited by: For subculturing: resuspend x 10 6 cells in 10 mL selection medium in a 75 cm 2 cell culture flask (cell density x 10 5 cells/mL) and incubate the cells at 37°C with 5 % CO 2.

Split the cells twice per Cited by: ced-4 Cell Death (pro-apopto tic B cl-2 members) (anti-apopto ic B l-2 members) (caspases, Apaf-1) Fig. 3 C. elegans as a model system contains basic components of the cell death machinery. Apoptosis. The cell densities achieved in perfusion culture (30– x 10 6 cells/mL) are typically higher than for fed-batch modes (5–25 x 10 6 cells/mL).

2 The principal aspect of perfusion operation. Compared to control mice, those that received the antibody showed massive amounts of nerve cell death. After a week of daily antibody injections, up to 99 percent of the neurons in certain parts of the.

The basic concepts were that the proapoptotic and antiapoptotic proteins balance each other with the more abundant protein dominating, pushing the cell towards apoptosis or survival. BAX was shown to. Hybridomas are finding increased use for the production of a wide variety of monoclonal antibodies.

Understanding the roles of physiological and environmental factors on the growtCited by:. Table 1 Carbon sources for the central metabolism of the CHO-K1 cell line in batch culture during the exponential growth phase Full size table The glutamine uptake flux determined by fitting eq.

Cited by:   Apoptosis can be defined as 'gene-directed cellular self-destruction'; it is sometimes referred to as 'programmed cell death' although this is really a phenomenon where cells are .This book is a collection of selected and relevant research, concerning the developments within the Cell Death field of study.

Each contribution comes as a separate chapter complete in itself but directly Cited by: 3.